{Reference Type}: Journal Article
{Title}: Exploiting the RUSH System to Study Lytic Granule Biogenesis in Cytotoxic T Lymphocytes.
{Author}: Capitani N;Cassioli C;Ravichandran K;Baldari CT;
{Journal}: Methods Mol Biol
{Volume}: 2654
{Issue}: 0
{Year}: 2023
暂无{DOI}: 10.1007/978-1-0716-3135-5_27
{Abstract}: The Retention Using Selective Hooks (RUSH) system allows for the synchronized release of one or more proteins of interest from a donor endomembrane compartment, usually the endoplasmic reticulum, and the subsequent monitoring of their traffic toward acceptor compartments. Here we describe the RUSH system applied to cytotoxic T cells to characterize the biogenesis of lytic granules, using as a proof-of-concept granzyme B trafficking to this specialized compartment.