{Reference Type}: Journal Article
{Title}: Monitoring the Release of Methylglyoxal (MGO) from Honey and Honey-Based Formulations.
{Author}: Hossain ML;Lim LY;Hammer K;Hettiarachchi D;Locher C;
{Journal}: Molecules
{Volume}: 28
{Issue}: 6
{Year}: Mar 2023 22
{Factor}: 4.927
{DOI}: 10.3390/molecules28062858
{Abstract}: Methylglyoxal (MGO) is considered to be one of the vital components responsible for the anti-bacterial activity of Leptospermum spp. (Manuka) honey. While many studies have demonstrated a dose-dependent antibacterial activity for MGO in vitro, from a therapeutic viewpoint, it is also important to confirm its release from Manuka honey and also from Manuka honey-based formulations. This study is the first to report on the release profile of MGO from five commercial products containing Manuka honey using a Franz diffusion cell and High-Performance Liquid Chromatography (HPLC) analysis. The release of MGO expressed as percentage release of MGO content at baseline was monitored over a 12 h period and found to be 99.49 and 98.05% from an artificial honey matrix and NZ Manuka honey, respectively. For the investigated formulations, a time-dependent % MGO release between 85% and 97.18% was noted over the 12 h study period.