{Reference Type}: Journal Article
{Title}: Identification of the viral and cellular microRNA interactomes during SARS-CoV-2 infection.
{Author}: Fossat N;Lundsgaard EA;Costa R;Rivera-Rangel LR;Nielsen L;Mikkelsen LS;Ramirez S;Bukh J;Scheel TKH;
{Journal}: Cell Rep
{Volume}: 42
{Issue}: 4
{Year}: 04 2023 25
暂无{DOI}: 10.1016/j.celrep.2023.112282
{Abstract}: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has had a tremendous impact worldwide. Mapping virus-host interactions is critical to understand disease progression. MicroRNAs (miRNAs) are important RNA regulators, but their interaction with SARS-CoV-2 RNA was not experimentally investigated. Here, using Argonaute (AGO) cross-linking immunoprecipitation combined with RNA proximity ligation (CLEAR-CLIP), we provide unbiased mapping of SARS-CoV-2/miRNA interactions. We identified six main regions on the viral RNA bound primarily by one specific miRNA. Targeted mutagenesis and AGO1-3 knockdown demonstrated that these interactions are not critical for virus production. Moreover, we identified perturbed regulation of cellular miRNA interactions during infection, including non-compensated viral sequestration of the miR-15 family. Transcriptome analysis further showed that mRNAs targeted by this miRNA family are derepressed. This work delineates the interphase between miRNA regulation and SARS-CoV-2 infection and further contributes to deciphering the full molecular interactome of this virus.