{Reference Type}: Journal Article
{Title}: Gene expression variability across cells and species shapes the relationship between renal resident macrophages and infiltrated macrophages.
{Author}: Ji X;Cai J;Liang L;Shi T;Liu J;
{Journal}: BMC Bioinformatics
{Volume}: 24
{Issue}: 1
{Year}: Mar 2023 1
{Factor}: 3.307
{DOI}: 10.1186/s12859-023-05198-z
{Abstract}: <B>BACKGROUND: </B>Two main subclasses of macrophages are found in almost all solid tissues: embryo-derived resident tissue macrophages and bone marrow-derived infiltrated macrophages. These macrophage subtypes show transcriptional and functional divergence, and the programs that have shaped the evolution of renal macrophages and related signaling pathways remain poorly understood. To clarify these processes, we performed data analysis based on single-cell transcriptional profiling of renal tissue-resident and infiltrated macrophages in human, mouse and rat.<BR><B>RESULTS: </B>In this study, we (i) characterized the transcriptional divergence among species and (ii) illustrated variability in expression among cells of each subtype and (iii) compared the gene regulation network and (iv) ligand-receptor pairs in human and mouse. Using single-cell transcriptomics, we mapped the promoter architecture during homeostasis.<BR><B>CONCLUSIONS: </B>Transcriptionally divergent genes, such as the differentially TF-encoding genes expressed in resident and infiltrated macrophages across the three species, vary among cells and include distinct promoter structures. The gene regulatory network in infiltrated macrophages shows comparatively better species-wide consistency than resident macrophages. The conserved transcriptional gene regulatory network in infiltrated macrophages among species is uniquely enriched in pathways related to kinases, and TFs associated with largely conserved regulons among species are uniquely enriched in kinase-related pathways.