{Reference Type}: Journal Article
{Title}: SLFN5-mediated chromatin dynamics sculpt higher-order DNA repair topology.
{Author}: Huang J;Wu C;Kloeber JA;Gao H;Gao M;Zhu Q;Chang Y;Zhao F;Guo G;Luo K;Dai H;Liu S;Huang Q;Kim W;Zhou Q;Zhu S;Wu Z;Tu X;Yin P;Deng M;Wang L;Yuan J;Lou Z;
{Journal}: Mol Cell
{Volume}: 83
{Issue}: 7
{Year}: 04 2023 6
{Factor}: 19.328
{DOI}: 10.1016/j.molcel.2023.02.004
{Abstract}: Repair of DNA double-strand breaks (DSBs) elicits three-dimensional (3D) chromatin topological changes. A recent finding reveals that 53BP1 assembles into a 3D chromatin topology pattern around DSBs. How this formation of a higher-order structure is configured and regulated remains enigmatic. Here, we report that SLFN5 is a critical factor for 53BP1 topological arrangement at DSBs. Using super-resolution imaging, we find that SLFN5 binds to 53BP1 chromatin domains to assemble a higher-order microdomain architecture by driving damaged chromatin dynamics at both DSBs and deprotected telomeres. Mechanistically, we propose that 53BP1 topology is shaped by two processes: (1) chromatin mobility driven by the SLFN5-LINC-microtubule axis and (2) the assembly of 53BP1 oligomers mediated by SLFN5. In mammals, SLFN5 deficiency disrupts the DSB repair topology and impairs non-homologous end joining, telomere fusions, class switch recombination, and sensitivity to poly (ADP-ribose) polymerase inhibitor. We establish a molecular mechanism that shapes higher-order chromatin topologies to safeguard genomic stability.