{Reference Type}: Journal Article
{Title}: Electron microscopy reveals toroidal shape of master neuronal cell differentiator REST - RE1-silencing transcription factor.
{Author}: Veverka P;Brom T;Janovič T;Stojaspal M;Pinkas M;Nováček J;Hofr C;
{Journal}: Comput Struct Biotechnol J
{Volume}: 21
{Issue}: 0
{Year}: 2023
{Factor}: 6.155
{DOI}: 10.1016/j.csbj.2022.12.026
{Abstract}: The RE1-Silencing Transcription factor (REST) is essential for neuronal differentiation. Here, we report the first 18.5-angstrom electron microscopy structure of human REST. The refined electron map suggests that REST forms a torus that can accommodate DNA double-helix in the central hole. Additionally, we quantitatively described REST binding to the canonical DNA sequence of the neuron-restrictive silencer element. We developed protocols for the expression and purification of full-length REST and the shortened variant REST-N62 produced by alternative splicing. We tested the mutual interaction of full-length REST and the splicing variant REST-N62. Revealed structure-function relationships of master neuronal repressor REST will allow finding new biological ways of prevention and treatment of neurodegenerative disorders and diseases.