{Reference Type}: Journal Article
{Title}: A high-throughput electron tomography workflow reveals over-elongated centrioles in relapsed/refractory multiple myeloma.
{Author}: Dittrich T;Köhrer S;Schorb M;Haberbosch I;Börmel M;Goldschmidt H;Pajor G;Müller-Tidow C;Raab MS;Hegenbart U;Schönland SO;Schwab Y;Krämer A;
{Journal}: Cell Rep Methods
{Volume}: 2
{Issue}: 11
{Year}: 11 2022 21
暂无{DOI}: 10.1016/j.crmeth.2022.100322
{Abstract}: Electron microscopy is the gold standard to characterize centrosomal ultrastructure. However, production of significant morphometrical data is highly limited by acquisition time. We therefore developed a generalizable, semi-automated high-throughput electron tomography strategy to study centrosome aberrations in sparse patient-derived cancer cells at nanoscale. As proof of principle, we present electron tomography data on 455 centrioles of CD138pos plasma cells from one patient with relapsed/refractory multiple myeloma and CD138neg bone marrow mononuclear cells from three healthy donors as a control. Plasma cells from the myeloma patient displayed 122 over-elongated centrioles (48.8%). Particularly mother centrioles also harbored gross structural abnormalities, including fragmentation and disturbed microtubule cylinder formation, while control centrioles were phenotypically unremarkable. These data demonstrate the feasibility of our scalable high-throughput electron tomography strategy to study structural centrosome aberrations in primary tumor cells. Moreover, our electron tomography workflow and data provide a resource for the characterization of cell organelles beyond centrosomes.