{Reference Type}: Journal Article
{Title}: Molecular mechanism of di-n-butyl phthalate promotion of bladder cancer development.
{Author}: Li EH;Xu BH;Wei HB;Bai YC;Zhang Q;Yu WW;Xu ZH;Qi XL;Zhang DH;Wang H;
{Journal}: Toxicol In Vitro
{Volume}: 86
{Issue}: 0
{Year}: Feb 2023
{Factor}: 3.685
{DOI}: 10.1016/j.tiv.2022.105508
{Abstract}: <B>OBJECTIVE: </B>To determine whether di-n-butyl phthalate (DBP) promotes the occurrence of bladder cancer (BCa) and explore the action of DBP acts on BCa cells at the cellular and molecular levels.<BR><B>METHODS: </B>MTT and Transwell assays were used to investigate the tumorigenic actions of DBP on BCa cells. Second-generation sequencing was used to identify differences in gene expression before and after DBP treatment. Differential gene expression was verified by q-PCR and analyzed using bioinformatics. Cells were transfected to overexpress genes of interest and proliferation and migration were measured using MTT and Transwell assays, respectively.<BR><B>RESULTS: </B>DBP treatment stimulated both proliferation and invasion in BCa cells. Second-generation sequencing identified differences in the expression of FOSB, JUND, ATP6V1C2, and RHOQ before and after DBP treatment. FOSB expression was confirmed by q-PCR and bioinformatic analyses. FOSB overexpression increased both proliferation and invasion in BCa cells.<BR><B>CONCLUSIONS: </B>DBP promoted BCa tumorigenesis by inducing changes in gene expression.