{Reference Type}: Journal Article
{Title}: Non-HLA Antibodies and Their Role in Highly Sensitized Patients.
{Author}: Roa-Bautista A;López-Del-Moral C;González-López E;Gutiérrez-Larrañaga M;Renuncio-García M;Castro-Hernández C;Mikhalkovich-Mikhalkovich D;Comins-Boo A;Irure-Ventura J;Rodrigo E;Ruiz San Millán JC;López-Hoyos M;San Segundo D;
{Journal}: Transplant Proc
{Volume}: 54
{Issue}: 9
{Year}: Nov 2022
{Factor}: 1.014
{DOI}: 10.1016/j.transproceed.2022.10.011
{Abstract}: BACKGROUND: The role of non-HLA antibody is gaining special attention in solid-organ transplantation and in highly sensitized (HS) patients because of its potential involvement in graft loss (GL) and/or antibody-mediated rejection (ABMR). The identification of non-HLA antibodies while listed may provide deeper information about the increased immunologic risk prior to transplant. We aimed to identify non-HLA antibodies pretransplant that could involve GL in HS patients.
METHODS: Nineteen pretransplant samples from HS patients who underwent transplant at the Marqués de Valdecilla University Hospital were studied for both HLA antibodies and a panel of 39 non-HLA antigens analyzed based on Luminex platform.
RESULTS: Eleven patient (57.9%) maintained the graft (KT group), whereas 8 (42.1%) had a GL within a median of 30 days. The median fluorescent intensity (MFI) of the 39 non-HLA antigens were compared within the groups, obtaining a statistically significant differences in protein tyrosine phosphatase receptor type N (P < .04) with a MFI mean of 1408 vs 4931 for KT and GL groups, respectively. However, no significant differences were observed in non-HLA MFI between ABMR and non-ABMR KT recipients.
CONCLUSIONS: The presence of non-HLA antibodies in HS is high. The levels of anti-protein tyrosine phosphatase receptor type N before transplant could indicate a potential risk of GL, although longitudinal studies with large number of cases are needed to define anti-non-HLA profiles of risk of ABMR.