{Reference Type}: Journal Article
{Title}: Cholesterol Chip for the Study of Cholesterol-Protein Interactions Using SPR.
{Author}: He P;Faris S;Sagabala RS;Datta P;Xu Z;Callahan B;Wang C;Boivin B;Zhang F;Linhardt RJ;
{Journal}: Biosensors (Basel)
{Volume}: 12
{Issue}: 10
{Year}: Sep 2022 25
{Factor}: 5.743
{DOI}: 10.3390/bios12100788
{Abstract}: Cholesterol, an important lipid in animal membranes, binds to hydrophobic pockets within many soluble proteins, transport proteins and membrane bound proteins. The study of cholesterol-protein interactions in aqueous solutions is complicated by cholesterol's low solubility and often requires organic co-solvents or surfactant additives. We report the synthesis of a biotinylated cholesterol and immobilization of this derivative on a streptavidin chip. Surface plasmon resonance (SPR) was then used to measure the kinetics of cholesterol interaction with cholesterol-binding proteins, hedgehog protein and tyrosine phosphatase 1B.