{Reference Type}: Journal Article {Title}: Isolation and Characterization of Peritoneal Macrophages from Salamanders. {Author}: Sallese A;Tsissios G;Pérez-Estrada JR;Martinez A;Del Rio-Tsonis K; {Journal}: Methods Mol Biol {Volume}: 2562 {Issue}: 0 {Year}: 2023 暂无{DOI}: 10.1007/978-1-0716-2659-7_18 {Abstract}: Salamanders have been used as research models for centuries. While they exhibit a wide range of biological features not seen in mammals, none has captivated scientists like their ability to regenerate. Interestingly, axolotl macrophages have emerged as an essential cell population for tissue regeneration. Whether the same is true in other salamanders such as newt species Notophthalmus viridescens, Cynops pyrrhogaster, or Pleurodeles waltl remains to be seen. Unfortunately, regardless of the species, molecular tools to study macrophage function in salamanders are lacking. We propose that the readily available, terminally differentiated peritoneal macrophages from newts or axolotls could be used to validate molecular reagents in the study of macrophage function during tissue regeneration in salamanders.