{Reference Type}: Journal Article
{Title}: Two-step förster resonance energy transfer amplification for ratiometric detection of pathogenic bacteria in food samples.
{Author}: Li Z;Xu H;Feng Q;Zhang Z;Miao X;
{Journal}: Food Chem
{Volume}: 404
{Issue}: 0
{Year}: Mar 2023 15
{Factor}: 9.231
{DOI}: 10.1016/j.foodchem.2022.134492
{Abstract}: We described a two-step förster resonance energy transfer (FRET) system for ratiometric Staphylococcus aureus (S. aureus) detection based on a dual-recognition proximity binding-induced toehold strand displacement reactions (TSDR). Ru(bpy)32+ and platinum nanoparticles (Pt NPs) labeled DNA (Ru-S3 and Pt NPs-S4) hybridized to enable the occurrence of the primary FRET using Ru(bpy)32+ as the energy donor and Pt NPs as the energy acceptor. TSDR happened by integrating vancomycin hydrochloride labeled S1 (Van-S1) and gold nanoclusters labeled S2-aptamer (Au NCs-S2-aptamer) with S. aureus. The single DNA segments of Van-S1 bond to the terminal toehold of Ru-S3, displacing Pt-S4, inducing the secondary FRET using Au NCs as the energy donor and Ru(bpy)32+ as the energy acceptor. This two-step FRET system efficiently improved the reaction efficiency of S. aureus with a detection limit of 1.0 CFU/mL. Furthermore, satisfactory results obtained while detecting S. aureus in food samples, indicating a great potential for food analysis.