{Reference Type}: Journal Article
{Title}: Simultaneous detection of foodborne pathogenic bacteria in milk by fluorescence immunoassay.
{Author}: Cheng X;Wang CC;Liu A;Liu Y;Dong J;Wang Z;Wei W;Liu S;
{Journal}: Spectrochim Acta A Mol Biomol Spectrosc
{Volume}: 285
{Issue}: 0
{Year}: Sep 2022 17
{Factor}: 4.831
{DOI}: 10.1016/j.saa.2022.121830
{Abstract}: Rapid, sensitive and simultaneous detection of multiple bacteria in foodborne is still a major challenge in public health field. Here, a fluorescence immunoassay that can achieve high-throughput detection of three Gram-positive foodborne pathogenic bacteria simultaneously was proposed. Vancomycin and bovine serum albumin conjugate (Van-BSA) was immobilized on a polycarbonate chip to capture three Gram-positive foodborne pathogenic bacteria, Staphylococcus aureus (S. aureus), Bacillus cereus (B. cereus) and Listeria monocytogenes (L. monocytogenes). CdSe/ZnS quantum dot modified antibodies (Ab-QD) were prepared by carbodiimide coupling chemistry. Due to the affinity reaction between antibodies and proteins on the bacterial surface, the simultaneous detection of multiple Gram-positive bacteria was achieved by monitoring the fluorescence signal of quantum dot by a portable microfluidic chip analyzer. Under optimal conditions, low detection limits was 18 CFU/well, 3 CFU/well and 36 CFU/well for S. aureus, B. cereus and L. monocytogenes, respectively. With satisfactory accuracy and precision, the proposed fluorescence immunoassay holds good prospects to detect pathogens in real food samples.