{Reference Type}: Journal Article
{Title}: New prenylated flavonoid glycosides derived from Epimedium wushanense by β-glucosidase hydrolysis and their testosterone production-promoting effects.
{Author}: Sun XG;Pang X;Liang HZ;Zhang J;Wang B;Li Q;Wang J;Chen XJ;Guo BL;Ma BP;Sun XG;Pang X;Liang HZ;Zhang J;Wang B;Li Q;Wang J;Chen XJ;Guo BL;Ma BP;
{Journal}: Chin J Nat Med
{Volume}: 20
{Issue}: 9
{Year}: Sep 2022
{Factor}: 3.887
{DOI}: 10.1016/S1875-5364(22)60188-2
{Abstract}: Six new prenylated flavonoid glycosides, including four new furan-flavonoid glycosides wushepimedoside A-D (1-4) and two new prenyl flavonoid derivatives wushepimedoside E-F (5-6), and one know analog epimedkoreside B (7) were isolated from biotransformation products of the aerial parts of Epimedium wushanense. Their structures were elucidated according to comprehensive analysis of HR-MS and NMR spectroscopic data, and the absolute configurations were assigned using experimental and calculated electronic circular dichroism (ECD) data. The regulatory activity of compounds 1-7 on the production of testosterone in primary rat Leydig cells were investigated, and 4 and 5 exhibited testosterone production-promoting activities. Molecular docking analysis suggested that bioactive compounds 4 and 5 showed the stable binding with 3β-HSD and 4 also had good affinity with Cyp17A1, which suggested that these compounds may regulate testosterone production through stimulating the expression of the above two key proteins.