{Reference Type}: Journal Article {Title}: Effects mechanism of bio-carrier filling rate on rotating biofilms and the reactor performance optimization method. {Author}: Wang JH;Zhao XL;Hu Q;Gao X;Qu B;Cheng Y;Feng D;Shi LF;Chen WH;Shen Y;Chen YP;Wang JH;Zhao XL;Hu Q;Gao X;Qu B;Cheng Y;Feng D;Shi LF;Chen WH;Shen Y;Chen YP; {Journal}: Chemosphere {Volume}: 308 {Issue}: 0 {Year}: Aug 2022 28 {Factor}: 8.943 {DOI}: 10.1016/j.chemosphere.2022.136176 {Abstract}: Benefited from the massive filling bio-carriers, the packed cage rotating biological contactors (RBCs) have better performance and application potentiality in wastewater treatment. Investigating the effects mechanism of bio-carrier filling rate is crucial for such reactors management. In this study, the pollutants removal performance, biofilms physical characteristics, and microbial communities of the biofilms under a series of bio-carrier filling rates were analyzed. The results shown, the pollutant removal rate and amount were quite different under different filling rates, and biofilms structure and microbial composition were the main factors affecting the pollutants removal performance. With the increasing filling rates, the biofilms were more mass increased (dry weight from 0.066 to 0.148 g/per carrier), thicker (from 340.30 to 850.84 μm) and lower dense (from 0.068 to 0.060 g/cm3). The microbial community composition of those biofilms was also quite different at the genus level. The effects mechanism of bio-carrier filling rate can be summarized: the filling rates affect the physical and biological characteristics of biofilms, which will further affect the microenvironment and microbial distribution in biofilms, and then determines the pollutant metabolic rate and metabolic pathway. This study will contribute to design better bio-carrier filling rate according to different wastewater treatment scenario, and promote the performance optimization of packed cage RBCs.