{Reference Type}: Journal Article
{Title}: GLP-1 regulates exercise endurance and skeletal muscle remodeling via GLP-1R/AMPK pathway.
{Author}: Wu L;Zhou M;Li T;Dong N;Yi L;Zhang Q;Mi M;Wu L;Zhou M;Li T;Dong N;Yi L;Zhang Q;Mi M;
{Journal}: Biochim Biophys Acta Mol Cell Res
{Volume}: 1869
{Issue}: 9
{Year}: Sep 2022
{Factor}: 5.011
{DOI}: 10.1016/j.bbamcr.2022.119300
{Abstract}: Exercise-induced physical endurance enhancement and skeletal muscle remodeling can prevent and delay the development of multiple diseases, especially metabolic syndrome. Herein, the study explored the association between glucagon-like peptide-1 (GLP-1) secretion and exercise, and its effect on skeletal muscle remodeling to enhance endurance capacity. We found both acute exercise and short-term endurance training significantly increased the secretion of GLP-1 in mice. Recombinant adeno-associated virus (AAV) encoding Gcg (proglucagon) was used to induce the overexpression of GLP-1 in skeletal muscle of mice. Overexpression of GLP-1 in skeletal muscle enhanced endurance capacity. Meanwhile, glycogen synthesis, glucose uptake, type I fibers proportion, and mitochondrial biogenesis were augmented in GLP-1-AAV skeletal muscle. Furthermore, the in vitro experiment showed that exendin-4 (a GLP-1 receptor agonist) treatment remarkably promoted glucose uptake, type I fibers formation, and mitochondrial respiration. Mechanistically, the knockdown of AMPK could reverse the effects imposed by GLP-1R activation in vitro. Taken together, these results verify that GLP-1 regulates skeletal muscle remodeling to enhance exercise endurance possibly via GLP-1R signaling-mediated phosphorylation of AMPK.