{Reference Type}: Journal Article
{Title}: Replication Labeling Methods for Super-Resolution Imaging of Chromosome Territories and Chromatin Domains.
{Author}: Miron E;Windo J;Ochs F;Schermelleh L;
{Journal}: Methods Mol Biol
{Volume}: 2476
{Issue}: 0
{Year}: 2022
暂无{DOI}: 10.1007/978-1-0716-2221-6_9
{Abstract}: Continuing progress in super-resolution microscopy enables the study of sub-chromosomal chromatin organization in single cells with unprecedented detail. Here we describe refined methods for pulse-chase replication labeling of individual chromosome territories (CTs) and replication domain units in mammalian cell nuclei, with specific focus on their application to three-dimensional structured illumination microscopy (3D-SIM). We provide detailed protocols for highly efficient electroporation-based delivery or scratch loading of cell-impermeable fluorescent nucleotides for live-cell studies. Furthermore, we describe the application of (2'S)-2'-deoxy-2'-fluoro-5-ethynyluridine (F-ara-EdU) and 5-vinyl-2'-deoxyuridine (VdU) for the in situ detection of segregated chromosome territories and sister chromatids with minimized cytotoxic side effects.