{Reference Type}: Journal Article
{Title}: Interactive, integrated analysis of single-cell transcriptomic and phylogenetic data with PhyloVision.
{Author}: Jones MG;Rosen Y;Yosef N;
{Journal}: Cell Rep Methods
{Volume}: 2
{Issue}: 4
{Year}: 04 2022 25
暂无{DOI}: 10.1016/j.crmeth.2022.100200
{Abstract}: Recent advances in CRISPR-Cas9 engineering and single-cell assays have enabled the simultaneous measurement of single-cell transcriptomic and phylogenetic profiles. However, there are few computational tools enabling users to integrate and derive insight from a joint analysis of these two modalities. Here, we describe "PhyloVision": an open-source software for interactively exploring data from both modalities and for identifying and interpreting heritable gene modules whose concerted expression are associated with phylogenetic relationships. PhyloVision provides a feature-rich, interactive, and shareable web-based report for investigating these modules while also supporting several other data and meta-data exploration capabilities. We demonstrate the utility of PhyloVision using a published dataset of metastatic lung adenocarcinoma cells, whose phylogeny was resolved using a CRISPR-Cas9-based lineage-tracing system. Together, we anticipate that PhyloVision and the methods it implements will be a useful resource for scalable and intuitive data exploration for any assay that simultaneously measures cell state and lineage.