{Reference Type}: Journal Article {Title}: Reply to Sheval, E.V. Comment on "Piña et al. Ten Approaches That Improve Immunostaining: A Review of the Latest Advances for the Optimization of Immunofluorescence. Int. J. Mol. Sci. 2022, 23, 1426". {Author}: Piña R;Acosta-Galeana I;Rosas-Arellano A; {Journal}: Int J Mol Sci {Volume}: 23 {Issue}: 8 {Year}: Apr 2022 15 {Factor}: 6.208 {DOI}: 10.3390/ijms23084375 {Abstract}: We have carefully read the interesting explanatory comment by Eugene V. Sheval [...].