{Reference Type}: Journal Article
{Title}: Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions.
{Author}: Lisacek F;
{Journal}: J Vis Exp
{Volume}: 0
{Issue}: 179
{Year}: 01 2022 20
{Factor}: 1.424
{DOI}: 10.3791/63356
{Abstract}: The Glyco@Expasy initiative was launched as a collection of interdependent databases and tools spanning several aspects of knowledge in glycobiology. In particular, it aims at highlighting interactions between glycoproteins (such as cell surface receptors) and carbohydrate-binding proteins mediated by glycans. Here, major resources of the collection are introduced through two illustrative examples centered on the N-glycome of the human Prostate Specific Antigen (PSA) and the O-glycome of human serum proteins. Through different database queries and with the help of visualization tools, this article shows how to explore and compare content in a continuum to gather and correlate otherwise scattered pieces of information. Collected data are destined to feed more elaborate scenarios of glycan function. Glycoinformatics introduced here is, therefore, proposed as a means to either strengthen, shape, or refute assumptions on the specificity of a protein glycome in a given context.