{Reference Type}: Journal Article
{Title}: [Preliminary study on the effect of B7H3 interaction with fibronectin on apoptosis of human chronic myeloid leukemia cells].
{Author}: Sun MY;Xie JJ;Zhang DZ;Zhang GB;
{Journal}: Zhonghua Xue Ye Xue Za Zhi
{Volume}: 42
{Issue}: 11
{Year}: Nov 2021 14
暂无{DOI}: 10.3760/cma.j.issn.0253-2727.2021.11.009
{Abstract}: Objective: To explore the effect of the interaction between B7H3 and fibronectin (FN) on the apoptosis of human chronic myeloid leukemia K562 cells. Methods: The expression of B7H3 molecules in K562 cells was detected using flow cytometry and B7H3 overexpressing cells were constructed. The interaction between B7H3 and FN was detected using the co-immunoprecipitation technology. After adding exogenous FN, cell experiments were performed to detect changes in adhesion and cell apoptosis. The changes in apoptosis-related proteins and PI3K/AKT signaling pathway were detected using Western blot. Results: The expression of B7H3 was low in K562, and the cell line K562 OE (overexpression) -B7H3 and the control cell line K562 NC (negative control) -B7H3 were obtained after lentivirus transfection. There is an interaction between B7H3 and FN (P=0.036) , and this interaction promoted cell adhesion (P<0.05) , inhibited cell apoptosis (P<0.05) , and activated the PI3K/AKT signaling pathway (P<0.05) . Conclusion: B7H3 interacts with FN to promote cell adhesion and may inhibit K562 cell apoptosis by activating the PI3K/AKT signaling pathway.<BR>目的： 探讨B7H3和纤维连接蛋白（FN）相互作用对人慢性髓性白血病细胞系K562细胞凋亡的影响。 方法： 采用流式细胞术检测K562细胞中B7H3分子的表达，构建B7H3过表达细胞。采用免疫共沉淀技术检测B7H3与FN的相互作用。添加外源FN后，通过细胞实验检测细胞黏附和细胞凋亡的变化。Western blot法检测凋亡相关蛋白及PI3K/AKT信号通路的变化。 结果： ① K562细胞低表达B7H3分子，慢病毒转染后得到稳定表达B7H3的细胞系K562 OE-B7H3及其对照细胞系K562 NC-B7H3细胞。②B7H3与FN之间存在相互作用（P=0.036）。③B7H3与FN的相互作用促进细胞黏附（P<0.05），抑制细胞凋亡（P<0.05）。④B7H3与FN相互作用激活PI3K/AKT信号通路（P<0.05）。 结论： B7H3与FN相互作用促进了细胞黏附，可能通过激活PI3K/AKT信号通路抑制K562细胞的凋亡。.