{Reference Type}: Journal Article
{Title}: Orai1 Boosts SK3 Channel Activation.
{Author}: Tiffner A;Hopl V;Schober R;Sallinger M;Grabmayr H;Höglinger C;Fahrner M;Lunz V;Maltan L;Frischauf I;Krivic D;Bhardwaj R;Schindl R;Hediger MA;Derler I;
{Journal}: Cancers (Basel)
{Volume}: 13
{Issue}: 24
{Year}: Dec 2021 17
{Factor}: 6.575
{DOI}: 10.3390/cancers13246357
{Abstract}: The interplay of SK3, a Ca2+ sensitive K+ ion channel, with Orai1, a Ca2+ ion channel, has been reported to increase cytosolic Ca2+ levels, thereby triggering proliferation of breast and colon cancer cells, although a molecular mechanism has remained elusive to date. We show in the current study, via heterologous protein expression, that Orai1 can enhance SK3 K+ currents, in addition to constitutively bound calmodulin (CaM). At low cytosolic Ca2+ levels that decrease SK3 K+ permeation, co-expressed Orai1 potentiates SK3 currents. This positive feedback mechanism of SK3 and Orai1 is enabled by their close co-localization. Remarkably, we discovered that loss of SK3 channel activity due to overexpressed CaM mutants could be restored by Orai1, likely via its interplay with the SK3-CaM binding site. Mapping for interaction sites within Orai1, we identified that the cytosolic strands and pore residues are critical for a functional communication with SK3. Moreover, STIM1 has a bimodal role in SK3-Orai1 regulation. Under physiological ionic conditions, STIM1 is able to impede SK3-Orai1 interplay by significantly decreasing their co-localization. Forced STIM1-Orai1 activity and associated Ca2+ influx promote SK3 K+ currents. The dynamic regulation of Orai1 to boost endogenous SK3 channels was also determined in the human prostate cancer cell line LNCaP.