{Reference Type}: Journal Article
{Title}: Study and Detection of Citrus Viroids in Woody Hosts.
{Author}: Krueger RR;Vidalakis G;
{Journal}: Methods Mol Biol
{Volume}: 2316
{Issue}: 0
{Year}: 2022
暂无{DOI}: 10.1007/978-1-0716-1464-8_1
{Abstract}: Biological indexing is based upon the ability of certain plants, referred to as indicator plants or indicators, to produce specific symptoms when inoculated with a pathogen using mechanical means or grafting. In the case of citrus viroids, clonal indicators are grafted on to vigorous rootstocks such as rough lemon (Citrus × granulata Raf.). The 'Arizona-861-S-1' citron clonal indicator (C. medica L.) can detect and bioamplify all citrus viroids; however, for specific citrus variants of the hop stunt viroid (i.e., CVd-IIb and CVd-IIc), the clonal indicator 'Parson's special # 9' mandarin (C. reticulata Blanco) is preferred. Inoculation techniques and symptom expression are described in detail. Other supporting elements, such as greenhouse conditions and propagation techniques, are also presented.