{Reference Type}: Journal Article
{Title}: Screening for the Key Proteins Associated with Rete Testis Invasion in Clinical Stage I Seminoma via Label-Free Quantitative Mass Spectrometry.
{Author}: Borszéková Pulzová L;Roška J;Kalman M;Kliment J;Slávik P;Smolková B;Goffa E;Jurkovičová D;Kulcsár Ľ;Lešková K;Bujdák P;Mego M;Bhide MR;Plank L;Chovanec M;
{Journal}: Cancers (Basel)
{Volume}: 13
{Issue}: 21
{Year}: Nov 2021 8
{Factor}: 6.575
{DOI}: 10.3390/cancers13215573
{Abstract}: Rete testis invasion (RTI) is an unfavourable prognostic factor for the risk of relapse in clinical stage I (CS I) seminoma patients. Notably, no evidence of difference in the proteome of RTI-positive vs. -negative CS I seminomas has been reported yet. Here, a quantitative proteomic approach was used to investigate RTI-associated proteins. 64 proteins were differentially expressed in RTI-positive compared to -negative CS I seminomas. Of them, 14-3-3γ, ezrin, filamin A, Parkinsonism-associated deglycase 7 (PARK7), vimentin and vinculin, were validated in CS I seminoma patient cohort. As shown by multivariate analysis controlling for clinical confounders, PARK7 and filamin A expression lowered the risk of RTI, while 14-3-3γ expression increased it. Therefore, we suggest that in real clinical biopsy specimens, the expression level of these proteins may reflect prognosis in CS I seminoma patients.