{Reference Type}: Journal Article
{Title}: Surfaceome CRISPR screen identifies OLFML3 as a rhinovirus-inducible IFN antagonist.
{Author}: Mei H;Zha Z;Wang W;Xie Y;Huang Y;Li W;Wei D;Zhang X;Qu J;Liu J;
{Journal}: Genome Biol
{Volume}: 22
{Issue}: 1
{Year}: 10 2021 22
暂无{DOI}: 10.1186/s13059-021-02513-w
{Abstract}: Rhinoviruses (RVs) cause more than half of common colds and, in some cases, more severe diseases. Functional genomics analyses of RVs using siRNA or genome-wide CRISPR screen uncovered a limited set of host factors, few of which have proven clinical relevance.<BR>Herein, we systematically compare genome-wide CRISPR screen and surface protein-focused CRISPR screen, referred to as surfaceome CRISPR screen, for their efficiencies in identifying RV host factors. We find that surfaceome screen outperforms the genome-wide screen in the success rate of hit identification. Importantly, using the surfaceome screen, we identify olfactomedin-like 3 (OLFML3) as a novel host factor of RV serotypes A and B, including a clinical isolate. We find that OLFML3 is a RV-inducible suppressor of the innate immune response and that OLFML3 antagonizes type I interferon (IFN) signaling in a SOCS3-dependent manner.<BR>Our study suggests that RV-induced OLFML3 expression is an important mechanism for RV to hijack the immune system and underscores surfaceome CRISPR screen in identifying viral host factors.