{Reference Type}: Journal Article
{Title}: Identification of Binding Proteins for TSC22D1 Family Proteins Using Mass Spectrometry.
{Author}: Kamimura R;Uchida D;Kanno SI;Shiraishi R;Hyodo T;Sawatani Y;Shimura M;Hasegawa T;Tsubura-Okubo M;Yaguchi E;Komiyama Y;Fukumoto C;Izumi S;Fujita A;Wakui T;Kawamata H;
{Journal}: Int J Mol Sci
{Volume}: 22
{Issue}: 20
{Year}: Oct 2021 9
{Factor}: 6.208
{DOI}: 10.3390/ijms222010913
{Abstract}: TSC-22 (TGF-β stimulated clone-22) has been reported to induce differentiation, growth inhibition, and apoptosis in various cells. TSC-22 is a member of a family in which many proteins are produced from four different family genes. TSC-22 (corresponding to TSC22D1-2) is composed of 144 amino acids translated from a short variant mRNA of the TSC22D1 gene. In this study, we attempted to determine the intracellular localizations of the TSC22D1 family proteins (TSC22D1-1, TSC-22 (TSC22D1-2), and TSC22(86) (TSC22D1-3)) and identify the binding proteins for TSC22D1 family proteins by mass spectrometry. We determined that TSC22D1-1 was mostly localized in the nucleus, TSC-22 (TSC22D1-2) was localized in the cytoplasm, mainly in the mitochondria and translocated from the cytoplasm to the nucleus after DNA damage, and TSC22(86) (TSC22D1-3) was localized in both the cytoplasm and nucleus. We identified multiple candidates of binding proteins for TSC22D1 family proteins in in vitro pull-down assays and in vivo binding assays. Histone H1 bound to TSC-22 (TSC22D1-2) or TSC22(86) (TSC22D1-3) in the nucleus. Guanine nucleotide-binding protein-like 3 (GNL3), which is also known as nucleostemin, bound to TSC-22 (TSC22D1-2) in the nucleus. Further investigation of the interaction of the candidate binding proteins with TSC22D1 family proteins would clarify the biological roles of TSC22D1 family proteins in several cell systems.