{Reference Type}: Journal Article
{Title}: Engineering threshold-based selection systems.
{Author}: Pedone KH;González-Pérez V;Leopold LE;Rasmussen NR;Der CJ;Cox AD;Ahmed S;Reiner DJ;
{Journal}: G3 (Bethesda)
{Volume}: 11
{Issue}: 9
{Year}: 09 2021 6
{Factor}: 3.542
{DOI}: 10.1093/g3journal/jkab234
{Abstract}: Using model organisms to identify novel therapeutic targets is frequently constrained by pre-existing genetic toolkits. To expedite positive selection for identification of novel downstream effectors, we engineered conditional expression of activated CED-10/Rac to disrupt Caenorhabditis elegans embryonic morphogenesis, titrated to 100% lethality. The strategy of engineering thresholds for positive selection using experimental animals was validated with pharmacological and genetic suppression and is generalizable to diverse molecular processes and experimental systems.