{Reference Type}: Journal Article
{Title}: Quantitation of vistusertib by UHPLC-MS/MS in rat plasma and its application to a pharmacokinetic study.
{Author}: Wang F;Song D;Zou F;Zhao H;Zhao X;
{Journal}: Bioanalysis
{Volume}: 13
{Issue}: 17
{Year}: Sep 2021
{Factor}: 2.695
{DOI}: 10.4155/bio-2021-0152
{Abstract}: Aim: The present study aimed to develop a UHPLC-MS/MS method for determination of vistusertib in biological matrix, and to describe the pharmacokinetic behavior of vistusertib in SD rats. Methodology & results: After protein precipitation with acetone and acetonitrile (1:1), the chromatographic separation was achieved on an Agilent Poroshell 120 EC-C18 column and detected with a SCIEX QTRAP 4500 mass spectrometer under positive ionization mode. The developed UHPLC-MS/MS method showed an excellent linearity within the range of 1.0-3000 ng/ml with good accuracy and precision. Vistusertib showed a rapid absorption and reached the maximum concentration of 3532.2 ± 678.0 ng/ml 20-30 min after oral administration in Sprague-Dawley rats. Conclusion: The established analytical method was fast, sensitive and robust, and successfully applied to describe the pharmacokinetic behavior of vistusertib following an oral administration in rats.