{Reference Type}: Journal Article
{Title}: Single-cell analysis of human skin identifies CD14+ type 3 dendritic cells co-producing IL1B and IL23A in psoriasis.
{Author}: Nakamizo S;Dutertre CA;Khalilnezhad A;Zhang XM;Lim S;Lum J;Koh G;Foong C;Yong PJA;Tan KJ;Sato R;Tomari K;Yvan-Charvet L;He H;Guttman-Yassky E;Malleret B;Shibuya R;Iwata M;Janela B;Goto T;Lucinda TS;Tang MBY;Theng C;Julia V;Hacini-Rachinel F;Kabashima K;Ginhoux F;
{Journal}: J Exp Med
{Volume}: 218
{Issue}: 9
{Year}: 09 2021 6
{Factor}: 17.579
{DOI}: 10.1084/jem.20202345
{Abstract}: Inflammatory skin diseases including atopic dermatitis (AD) and psoriasis (PSO) are underpinned by dendritic cell (DC)-mediated T cell responses. Currently, the heterogeneous human cutaneous DC population is incompletely characterized, and its contribution to these diseases remains unclear. Here, we performed index-sorted single-cell flow cytometry and RNA sequencing of lesional and nonlesional AD and PSO skin to identify macrophages and all DC subsets, including the newly described mature LAMP3+BIRC3+ DCs enriched in immunoregulatory molecules (mregDC) and CD14+ DC3. By integrating our indexed data with published skin datasets, we generated a myeloid cell universe of DC and macrophage subsets in healthy and diseased skin. Importantly, we found that CD14+ DC3s increased in PSO lesional skin and co-produced IL1B and IL23A, which are pathological in PSO. Our study comprehensively describes the molecular characteristics of macrophages and DC subsets in AD and PSO at single-cell resolution, and identifies CD14+ DC3s as potential promoters of inflammation in PSO.