{Reference Type}: Journal Article
{Title}: A new method of primary engineering of esophagus using orthotopic in-body tissue architecture.
{Author}: Suzuki K;Komura M;Obana K;Komura H;Inaki R;Fujishiro J;Suzuki K;Nakayama Y;
{Journal}: J Pediatr Surg
{Volume}: 56
{Issue}: 7
{Year}: Jul 2021
{Factor}: 2.549
{DOI}: 10.1016/j.jpedsurg.2021.03.025
{Abstract}: <B>OBJECTIVE: </B>Tissue engineering of esophagus is required for management of long-gap esophageal atresia (LGEA). Collagenous connective tissue membranes fabricated by in-body tissue architecture (iBTA), called biosheets, can repair esophageal defects and generate tissues similar to native esophagus. However, iBTA requires second-stage surgery because of heterotopic preparation of biosheets. Our aim was to develop orthotopic iBTA for primary engineering of the esophagus by interposing a tubular mold to the esophageal defect.<BR><B>METHODS: </B>The cervical esophagus of six rats was transected. An acrylic tube (internal diameter 2.6 mm, length 7.0 mm) was inserted and fixed between the ends of the upper and lower esophagus, and a 3 mm-long esophageal defect was created. Four weeks later, the rats were sacrificed for histological analysis.<BR><B>RESULTS: </B>Postoperatively the rats could intake liquid food. After four weeks, the esophageal defects were filled with regenerated tissues. Histologically the new esophageal walls stained positive for collagen type I. The inner surfaces were covered with stratified squamous epithelium that expressed pan-cytokeratin. In only one of six rats, regeneration of muscular-like tissue was suggested by positive immunohistochemical staining for desmin.<BR><B>CONCLUSIONS: </B>Orthotopic iBTA can regenerate a substitute esophagus with esophageal epithelium and collagenous wall. This technique may be a novel treatment for esophageal atresia with gaps of various lengths including LGEA.