{Reference Type}: Journal Article
{Title}: Association of FTO gene methylation with incident type 2 diabetes mellitus: A nested case-control study.
{Author}: Huang S;Qin P;Chen Q;Zhang D;Cheng C;Guo C;Li Q;Zhou Q;Tian G;Qie R;Han M;Wu X;Yang X;Feng Y;Li Y;Zhang Y;Wu Y;Liu D;Lu J;Zhang M;Zhao Y;Hu D;
{Journal}: Gene
{Volume}: 786
{Issue}: 0
{Year}: Jun 2021 20
{Factor}: 3.913
{DOI}: 10.1016/j.gene.2021.145585
{Abstract}: <B>OBJECTIVE: </B>This study aimed to investigate the association of FTO methylation level with type 2 diabetes mellitus (T2DM) in a nested case-control study.<BR><B>METHODS: </B>This nested case-control study included 287 pairs of T2DM cases and controls identified from a rural Chinese cohort study with a 6-year follow-up. Controls were matched to the cases on a 1:1 basis by age, sex, ethnicity, marital status, and residence. Conditional multivariate logistic regression models were used to calculate odds ratios (ORs) and 95% confidence intervals (CIs) for the association of cytosine guanine (CpG) locus and tag-single nucleotide polymorphisms (Tag-SNPs) with T2DM. Spearman correlation analysis was used to evaluate the association between FTO methylation and possible risk factors for T2DM in the control group.<BR><B>RESULTS: </B>The methylation level on the CpG9 site significantly differs between cases and controls, with a significant association between the CpG9 site methylation and probability of T2DM: OR 2.19 (95%CI: 1.31-3.65) after adjusting for potential confounders. The Tag-SNPs (rs72803657, rs1558902, rs17817449, rs11076023) were not associated with T2DM. Further, FTO methylation was associated with some risk factors for T2DM.<BR><B>CONCLUSIONS: </B>A CpG locus of FTO was positively associated with T2DM, but SNPs were not. FTO methylation were also associated with some T2DM risk factors. Further study with a large sample size and data on metabolic product are needed to confirm the association.