{Reference Type}: Journal Article {Title}: Life cycle assessment of urban wastewater treatment plants: a critical analysis and guideline proposal. {Author}: Rebello TA;Roque RP;Gonçalves RF;Calmon JL;Queiroz LM; {Journal}: Water Sci Technol {Volume}: 83 {Issue}: 3 {Year}: Feb 2021 {Factor}: 2.43 {DOI}: 10.2166/wst.2020.608 {Abstract}: In its 30 years of existence, there are still many improvement possibilities in studies performing the life cycle assessment (LCA) of wastewater treatment plants (WWTPs). Hence, this paper aims to start a guideline development for LCA of urban WWTPs based on the information available in the scientific literature on the topic. The authors used the ProKnow-C systematic review methodology for paper selection and 111 studies were analyzed. The most significant points that can be improved are caused by missing essential information (e.g. functional unity and input data). Other important methodological aspects are covered: allocation process, functional unit choice, sensitivity analysis, and important fluxes to be considered. Many opportunities within the LCA of WWTPs were identified, such as optimization of WWTP operational aspects and resource recovery. Furthermore, LCA should be combined with other methodologies such as big data, data envelopment analysis, life cycle cost assessment, and social life cycle assessment. To achieve this potential, it is clear that the scientific and technical community needs to converge on a new protocol to ensure that LCA application becomes more reliable and transparent.