{Reference Type}: Journal Article
{Title}: Variation under domestication in animal models: the case of the Mexican axolotl.
{Author}: Torres-Sánchez M;
{Journal}: BMC Genomics
{Volume}: 21
{Issue}: 1
{Year}: Nov 2020 23
{Factor}: 4.547
{DOI}: 10.1186/s12864-020-07248-9
{Abstract}: <B>BACKGROUND: </B>Species adaptation to laboratory conditions is a special case of domestication that has modified model organisms phenotypically and genetically. The characterisation of these changes is crucial to understand how this variation can affect the outcome of biological experiments. Yet despite the wide use of laboratory animals in biological research, knowledge of the genetic diversity within and between different strains and populations of some animal models is still scarce. This is particularly the case of the Mexican axolotl, which has been bred in captivity since 1864.<BR><B>RESULTS: </B>Using gene expression data from nine different projects, nucleotide sequence variants were characterised, and distinctive genetic background of the experimental specimens was uncovered. This study provides a catalogue of thousands of nucleotide variants along predicted protein-coding genes, while identifying genome-wide differences between pigment phenotypes in laboratory populations.<BR><B>CONCLUSIONS: </B>Awareness of the genetic variation could guide a better experimental design while helping to develop molecular tools for monitoring genetic diversity and studying gene functions in laboratory axolotls. Overall, this study highlights the cross-taxa utility that transcriptomic data might have to assess the genetic variation of the experimental specimens, which might help to shorten the journey towards reproducible research.