{Reference Type}: Journal Article
{Title}: Identification and broth-microdilution antifungal susceptibility testing of yeast directly from automated blood cultures.
{Author}: Das S;Tawde Y;Singh S;Chakrabarti A;Ray P;Rudramurthy SM;Kaur H;Ghosh A;
{Journal}: Future Microbiol
{Volume}: 0
{Issue}: 0
{Year}: Nov 2020 12
{Factor}: 3.553
{DOI}: 10.2217/fmb-2020-0077
{Abstract}: Aim: To standardize MALDI-TOF-MS based identification and antifungal susceptibility (AFST) for yeasts directly from automated blood cultures to reduce turnaround time. Materials & methods: Direct-ID after lysis-centrifugation (0.5% SDS) standardized in 40 and validated in 250 yeast positive samples. Direct-AFST was standardized with fluconazole (28 samples) and evaluated (70 samples) for seven antifungals. Results: Direct-ID had a high sensitivity (97.2%) and specificity (94.3%). Correct species-level identification showed 100% in C. tropicalis, C. krusei, C. parapsilosis. Direct-AFST had a 100% categorical agreement with culture-AFST for posaconazole, anidulafungin and >90% categorical agreement for amphotericin B, voriconazole and fluconazole. Conclusion: Direct-ID and subsequent direct-AFST is a rapid and robust method to reduce the turnaround time for the diagnosis of invasive candidiasis.