{Reference Type}: Journal Article
{Title}: Photoactivatable Fluorophore for Stimulated Emission Depletion (STED) Microscopy and Bioconjugation Technique for Hydrophobic Labels.
{Author}: Weber M;Khan TA;Patalag LJ;Bossi M;Leutenegger M;Belov VN;Hell SW;
{Journal}: Chemistry
{Volume}: 27
{Issue}: 1
{Year}: Jan 2021 4
{Factor}: 5.02
{DOI}: 10.1002/chem.202004645
{Abstract}: The use of photoactivatable dyes in STED microscopy has so far been limited by two-photon activation through the STED beam and by the fact that photoactivatable dyes are poorly solvable in water. Herein, we report ONB-2SiR, a fluorophore that can be both photoactivated in the UV and specifically de-excited by STED at 775 nm. Likewise, we introduce a conjugation and purification protocol to effectively label primary and secondary antibodies with moderately water-soluble dyes. Greatly reducing dye aggregation, our technique provides a defined and tunable degree of labeling, and improves the imaging performance of dye conjugates in general.