{Reference Type}: Journal Article
{Title}: Development of a method to quantify endogenous IFNγ protein in amberjack species.
{Author}: Matsuura Y;Takano T;Matsuyama T;Sakai T;Terashima S;Nakayasu C;
{Journal}: Fish Shellfish Immunol
{Volume}: 107
{Issue}: 0
{Year}: Dec 2020
{Factor}: 4.622
{DOI}: 10.1016/j.fsi.2020.10.013
{Abstract}: Interferon (IFN)γ is a pivotal cytokine that promotes and orchestrates innate cellular and adaptive cell-mediated immunity against intracellular pathogens. The capacity of T cells in mammals to produce IFNγ has been measured using specific antibodies in order to analyze cell-mediated immune responses against infection or immuno-stimulants. In fish, however, measurement of IFNγ protein levels has not been possible due to a lack of research tools. In the present study, therefore, we established antibodies that react with endogenous amberjack IFNγ. An enzyme-linked immunosorbent assay (ELISA) for IFNγ in amberjack species was developed using these antibodies. The ELISA could detect endogenous IFNγ at concentrations less than 100 pg/mL in PMA/ionomycin-stimulated leukocytes culture supernatant. IFNγ production was enhanced and lasted a long time following intracellular bacterial infection with Nocardia seriolae, which is thought to be targeted by cell-mediated immunity. These results demonstrate that quantification of IFNγ using the reported ELISA can be used to estimate the status of cell-mediated immunity in amberjack species.