{Reference Type}: Journal Article
{Title}: Quantitation of 2-hydroxyglutarate in human plasma via LC-MS/MS using a surrogate analyte approach.
{Author}: Yin F;Ling Y;Keller J;Kraus D;Narayanaswamy R;Mangus H;Li F;Yang H;Liu G;
{Journal}: Bioanalysis
{Volume}: 12
{Issue}: 16
{Year}: Aug 2020
{Factor}: 2.695
{DOI}: 10.4155/bio-2020-0131
{Abstract}: Aim: 2-Hydroxyglutarate (2-HG) is a target engagement biomarker in patients after treatment with inhibitors of mutated isocitrate dehydrogenase (mIDH). Accurate measurement of 2-HG is critical for monitoring the inhibition effectiveness of the inhibitors. Materials & methods: Human plasma samples were spiked with stable isotope labelled internal standard, processed by protein precipitation, and analyzed using LC-MS/MS. This method was validated following regulatory guidance and has been successfully applied in a clinical study for mIDH inhibition. Results: An LC-MS/MS method with a surrogate analyte approach was developed and validated to measure 2-HG in human plasma with acceptable intra- and inter-assay accuracy and precision. Conclusion: A sensitive and robust LC-MS/MS method was developed and validated for measuring 2-HG in human plasma.