{Reference Type}: Journal Article
{Title}: Transcriptional and Functional Analysis of CD1c+ Human Dendritic Cells Identifies a CD163+ Subset Priming CD8+CD103+ T Cells.
{Author}: Bourdely P;Anselmi G;Vaivode K;Ramos RN;Missolo-Koussou Y;Hidalgo S;Tosselo J;Nuñez N;Richer W;Vincent-Salomon A;Saxena A;Wood K;Lladser A;Piaggio E;Helft J;Guermonprez P;
{Journal}: Immunity
{Volume}: 53
{Issue}: 2
{Year}: 08 2020 18
{Factor}: 43.474
{DOI}: 10.1016/j.immuni.2020.06.002
{Abstract}: Dendritic cells (DCs) are antigen-presenting cells controlling T cell activation. In humans, the diversity, ontogeny, and functional capabilities of DC subsets are not fully understood. Here, we identified circulating CD88-CD1c+CD163+ DCs (called DC3s) as immediate precursors of inflammatory CD88-CD14+CD1c+CD163+FcεRI+ DCs. DC3s develop via a specific pathway activated by GM-CSF, independent of cDC-restricted (CDP) and monocyte-restricted (cMoP) progenitors. Like classical DCs but unlike monocytes, DC3s drove activation of naive T cells. In vitro, DC3s displayed a distinctive ability to prime CD8+ T cells expressing a tissue homing signature and the epithelial homing alpha-E integrin (CD103) through transforming growth factor β (TGF-β) signaling. In vivo, DC3s infiltrated luminal breast cancer primary tumors, and DC3 infiltration correlated positively with CD8+CD103+CD69+ tissue-resident memory T cells. Together, these findings define DC3s as a lineage of inflammatory DCs endowed with a strong potential to regulate tumor immunity.