{Reference Type}: Journal Article
{Title}: shRNA knockdown of integrin-linked kinase on hPDLCs migration, proliferation, and apoptosis under cyclic tensile stress.
{Author}: Wang Y;Du C;Wan W;He C;Wu S;Wang T;Wang F;Zou R;
{Journal}: Oral Dis
{Volume}: 26
{Issue}: 8
{Year}: Nov 2020
{Factor}: 4.068
{DOI}: 10.1111/odi.13474
{Abstract}: <B>OBJECTIVE: </B>To investigate the roles of integrin-linked kinase (ILK) in mediating the cell migration, proliferation, and apoptosis of human periodontal ligament cells (hPDLCs) in response to cyclic tensile stress.<BR><B>METHODS: </B>Primary hPDLCs were obtained through the enzyme digestion and tissue culture method. Short hairpin ILK-expressing hPDLCs were constructed using a recombinant lentiviral vector that specifically targeted ILK gene expression. The silencing of the ILK gene was identified by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. The hPDLCs were seeded on a flexible substrate and loaded with cyclic tensile stress at 0.5 Hz for 0, 2, 4, and 8 hr, consecutively, with the Flexcell Tension System. The response of cell migration was tested by the scratch assay. Cell proliferation was characterized by optical density (OD) value of cell counting kit-8 (CCK-8) test and Ki67 mRNA expression of qRT-PCR. Cell apoptosis was determined by flow cytometry and Caspase-3 mRNA expression of qRT-PCR.<BR><B>RESULTS: </B>Knocking down ILK substantially reduces migration and proliferation as well as regulates the sensitivity of hPDLCs to apoptosis under cyclic tensile stress.<BR><B>CONCLUSIONS: </B>ILK can promote the proliferation and migration as well as inhibit apoptosis of hPDLCs under cyclic tensile stress.