{Reference Type}: Journal Article
{Title}: Simple and Rapid Detection of Glycoforms by "Lectin Inhibition" Assay.
{Author}: Hoshi K;Hashimoto Y;Ito H;
{Journal}: Methods Mol Biol
{Volume}: 2132
{Issue}: 0
{Year}: 2020
暂无{DOI}: 10.1007/978-1-0716-0430-4_17
{Abstract}: Glycoforms are otherwise identical proteins with different glycosylation. A lectin, Sambucus sieboldiana agglutinin (SSA), specifically binds glycoforms having α2,6-sialyl residues. The binding is found to inhibit antigen-antibody reaction; e.g., SSA inhibits anti-transferrin antibody binding to α2,6-sialylated transferrin (Tf) (SSA inhibition). SSA inhibition is not observed with other Tf glycoforms, indicating that the inhibition is glycoform-specific. Here we describe the application of SSA inhibition to ELISA as a specific assay for quantifying α2,6-sialylated Tf.