{Reference Type}: Journal Article
{Title}: Isolating and Culturing Mouse Podocyte Cells to Study Diabetic Nephropathy.
{Author}: Vasilopoulou E;
{Journal}: Methods Mol Biol
{Volume}: 2067
{Issue}: 0
{Year}: 2020
暂无{DOI}: 10.1007/978-1-4939-9841-8_5
{Abstract}: Diabetic nephropathy is associated with injury and loss of podocytes, specialized epithelial cells that are critical for glomerular filtration. This chapter describes a method of isolating and culturing podocyte cells from mouse adult kidneys. In this way, podocytes with genetic modifications can be obtained from transgenic animals and they can be used to study the effects of the diabetic environment in vitro.