{Reference Type}: Journal Article {Title}: Producing Oleaginous Microorganisms Using Wastewater: Methods and Guidelines for Lab- and Industrial-Scale Production. {Author}: Rude KM;Barzee TJ;Franz AK; {Journal}: Methods Mol Biol {Volume}: 1995 {Issue}: 0 {Year}: 2019 暂无{DOI}: 10.1007/978-1-4939-9484-7_19 {Abstract}: Cultivation of oleaginous microorganisms on wastewater provides alternative biofuel options while also acting as a remediation technique for alternative wastewater treatment. This chapter describes guidelines and methods for the production of oleaginous microorganisms-with a focus on microalgae-using wastewater as a growth medium while considering a variety of general challenges for both lab- and industrial-scale production. Cultivation techniques described here range in scale from microplates with 10-mL working volumes, up to multigallon, industrial-scale microorganism cultivation, with a focus on microalgae. This chapter includes guidelines for the preparation of wastewater and selection of oleaginous microorganisms combined with methods for the production of oleaginous microorganisms cultivated using wastewater.