{Reference Type}: Journal Article
{Title}: A Fast and Reliable Method to Isolate Human Placental Macrophages.
{Author}: Mezouar S;Ben Amara A;Chartier C;Gorvel L;Mege JL;
{Journal}: Curr Protoc Immunol
{Volume}: 125
{Issue}: 1
{Year}: 06 2019
暂无{DOI}: 10.1002/cpim.77
{Abstract}: Macrophages are specialized cells involved in recognition, uptake, and destruction of microorganisms. Human placental macrophages are poorly investigated because of the lack of a convenient protocol for their isolation. Here, we present a straightforward and reliable method to isolate macrophages from full-term human placentas. After enzymatic digestion of placental tissue and centrifugation of the cell suspension on a Ficoll cushion, placental macrophages are selected using magnetic beads coated with anti-CD14 antibodies. Isolated cells are characterized by flow cytometry. Ninety eight percent of isolated CD14+ placental macrophages also express the macrophage marker CD68. Thus, this efficient and reliable method yields placental macrophages at high purity and sufficient quantity for functional studies. © 2019 by John Wiley & Sons, Inc.