{Reference Type}: Journal Article
{Title}: Three-Dimensional Reconstruction Imaging Method to Study the Function of EFHD2 in Invadopodia Formation.
{Author}: Huh YH;Yeo YR;Song WK;
{Journal}: Methods Mol Biol
{Volume}: 1929
{Issue}: 0
{Year}: 2019
暂无{DOI}: 10.1007/978-1-4939-9030-6_38
{Abstract}: Calcium and calcium-binding proteins play crucial roles in the regulation of actin dynamics, which contributes to cancer cell migration and invasion. In this chapter, we have focused on a three-dimensional imaging method to explore the pathophysiological function of EF-hand domain-containing protein D2 (EFHD2), a novel actin-binding protein. To overcome the limitations of two-dimensional imaging on substrate-coated cover glass for examination of invasive protrusions of cancer cells, we suggest three-dimensional reconstruction from optical z-sections of cells cultured on substrate-impregnated membrane filters of Transwell.