{Reference Type}: Journal Article
{Title}: Differential expression of cyclin E, p63, and Ki-67 in gestational trophoblastic disease and its role in diagnosis and management: A prospective case-control study.
{Author}: Kar A;Mishra C;Biswal P;Kar T;Panda S;Naik S;
{Journal}: Indian J Pathol Microbiol
{Volume}: 62
{Issue}: 1
{Year}: Jan-Mar 2019
{Factor}: 0.773
{DOI}: 10.4103/IJPM.IJPM_82_18
{Abstract}: <B>BACKGROUND: </B>Gestational trophoblastic disease (GTD) constitutes a spectrum of tumors and tumor-like conditions, characterized by proliferation of pregnancy-associated trophoblastic tissue of progressive malignant potential. It is very difficult to differentiate these complex groups of lesions basing on histomorphology alone. Immunohistochemistry (IHC) with cyclin E, P63, and Ki-67 has a definite role in the identification of different trophoblasts and entities of GTD and also in the determination of biological behavior.<BR><B>OBJECTIVE: </B>The aim of this study is to find the differential expression of cyclin E, p63, and Ki-67 in normal placenta, hydropic abortus (HA), and various entities of GTD.<BR><B>METHODS: </B>A prospective case-control study conducted in a government medical college.<BR><B>METHODS: </B>Total 96 cases, divided into Group A (48 histologically confirmed cases of GTD) and Group B (controls comprising 8 HA and 40 normal placentas of different trimesters), were studied. The histological samples were subjected to IHC using cyclin E, Ki-67, and p63.<BR><B>METHODS: </B>Results were analyzed using SPSS statistical method.<BR><B>RESULTS: </B>Among the three immunomarkers used, Cyclin E and Ki-67 show statistically significant difference (P < 0.05) when compared between GTD and control groups, but it was insignificant for p63 (P = 0.369). Strong staining intensity of cyclin E and Ki-67 is seen in complete moles, choriocarcinoma, and placental site trophoblastic tumor.<BR><B>CONCLUSIONS: </B>This study was done to evaluate the role of cell cycle regulatory proteins such as cyclin E and p63 and proliferation marker Ki-67 in the detection of various trophoblasts and differential diagnosis of the lesions associated with them.