{Reference Type}: Journal Article
{Title}: Muscle activity prevents the uncoupling of mitochondria from Ca2+ Release Units induced by ageing and disuse.
{Author}: Pietrangelo L;Michelucci A;Ambrogini P;Sartini S;Guarnier FA;Fusella A;Zamparo I;Mammucari C;Protasi F;Boncompagni S;
{Journal}: Arch Biochem Biophys
{Volume}: 663
{Issue}: 0
{Year}: 03 2019 15
{Factor}: 4.114
{DOI}: 10.1016/j.abb.2018.12.017
{Abstract}: In fast-twitch fibers from adult mice Ca2+ release units (CRUs, i.e. intracellular junctions of excitation-contraction coupling), and mitochondria are structurally linked to each other by small strands, named tethers. We recently showed that aging causes separation of a fraction of mitochondria from CRUs and a consequent impairment of the Ca2+ signaling between the two organelles. However, whether the uncoupling of mitochondria from CRUs is the result of aging per-se or the consequence of reduced muscle activity remains still unclear. Here we studied the association between mitochondria and CRUs: in a) extensor digitorum longus (EDL) muscles from 2 years old mice, either sedentary or trained for 1 year in wheel cages; and b) denervated EDL muscles from adult mice and rats. We analyzed muscle samples using a combination of structural (confocal and electron microscopy), biochemical (assessment of oxidative stress via western blot), and functional (ex-vivo contractile properties, and mitochondrial Ca2+ uptake) experimental procedures. The results collected in structural studies indicate that: a) ageing and denervation result in partial uncoupling between mitochondria and CRUs; b) exercise either maintains (in old mice) or restores (in transiently denervated rats) the association between the two organelles. Functional studies supported the hypothesis that CRU-mitochondria coupling is important for mitochondrial Ca2+ uptake, optimal force generation, and muscle performance. Taken together our results indicate that muscle activity maintains/improves proper association between CRUs and mitochondria.