{Reference Type}: Journal Article
{Title}: The synthesis of novel chromogenic enzyme substrates for detection of bacterial glycosidases and their applications in diagnostic microbiology.
{Author}: Burton M;Perry JD;Stanforth SP;Turner HJ;
{Journal}: Bioorg Med Chem
{Volume}: 26
{Issue}: 17
{Year}: 09 2018 15
{Factor}: 3.461
{DOI}: 10.1016/j.bmc.2018.08.023
{Abstract}: The preparation and evaluation of chromogenic substrates for detecting bacterial glycosidase enzymes is reported. These substrates are monoglycoside derivatives of the metal chelators catechol, 2,3-dihydroxynaphthalene (DHN) and 6,7-dibromo-2,3-dihydroxynaphthalene (6,7-dibromo-DHN). When hydrolysed by appropriate bacterial enzymes these substrates produced coloured chelates in the presence of ammonium iron(III) citrate, thus enabling bacterial detection. A β-d-riboside of DHN and a β-d-glucuronide derivative of 6,7-dibromo-DHN were particularly effective for the detection of S. aureus and E. coli respectively.