{Reference Type}: Journal Article
{Title}: [Preparation of multifunctional nanoparticles targeting tongue cancer and in vitro study].
{Author}: Ren W;Qiu LH;Gao Z;Li P;Zhao X;Hu CC;
{Journal}: Hua Xi Kou Qiang Yi Xue Za Zhi
{Volume}: 36
{Issue}: 3
{Year}: Jun 2018 1
暂无{DOI}: 10.7518/hxkq.2018.03.002
{Abstract}: <B>OBJECTIVE: </B>This study aims to prepare docetaxel (DOC)-loaded multifunctional nanoparticles containing indocyanine green (ICG) and perfluorohexane (PFH) as targeted drug delivery system, which is supplemented with stromal cellderived factor-1 (SDF-1), and characterize their properties.<BR><B>METHODS: </B>Multifunctional nanoparticles were prepared by using the double emulsion method. SDF-1 was covalently conjugated to the surface of the nanoparticles through thioether bonding. Their particle size, distribution, and surface potential were determined with the Malvern measuring instrument. The conjugation of SDF-1 was evaluated by confocal laser scanning microscope. Encapsulation efficiency (ELC), drug loading capacity (DLC), and release regularity of the nanoparticles were determined by high-performance liquid chromatography (HPLC). In vitro photothermal property was recorded by a thermal imager. The in vitro imaging capacity was observed by a photoacoustic instrument and an ultrasonic diagnostic apparatus. Targeting capability was assessed by flow cytometry. The cell activity on SCC-15 cells was checked by CCK-8 method.<BR><B>RESULTS: </B>The targeted multifunctional nanoparticles showed regularly sphericity. The diameter was (502.88±17.92) nm. The zeta potential was (-11.5±3.15) mV. ELC was 54.12%±1.74%. DLC was 1.08 mg·mL-1. In vitro drug release was initially fast and subsequently slow. The photothermal characteristics were related to the concentration; the higher the concentration, the higher the temperature. Nanoparticles could detect significant photoacoustic and ultrasound signals. The in vitro targeting rate was 89.99%. No significant differences of cell viability in the SINPs groups were observed at each concentration (P>0.05). The inhibition effect of DOC-SINPs was stronger than that of SINPs whether or not in the presence of laser irradiation among the groups of 150 and 200 μg·mL-1 (P<
0.05).<BR><B>CONCLUSIONS: </B>Multifunctional nanoparticles for diagnosis and treatment were successfully prepared and displayed dualmode ultrasound/photoacoustic imaging and antitumor effects of chemotherapy and photothermal therapy.<BR>目的 制备一种基质细胞衍生因子-1（SDF-1）修饰的载多西紫杉醇（DOC）包裹吲哚菁绿（ICG）和液态氟碳全氟己烷（PFH）靶向多功能纳米粒（DOC-SINPs），检测其性质。方法 双乳化法制备纳米粒，硫醚键连接SDF-1，得到靶向纳米粒。Malvern粒径仪检测其粒径及表面电位，激光扫描共聚焦显微镜观察SDF-1在纳米粒表面的连接情况，高效液相色谱法（HPLC）测定其DOC包封率（ELC）和载药量（DLC）及体外释放规律，热成像仪记录其体外光热特性，光声仪及超声诊断仪观察其体外显像，流式细胞仪评估其体外靶向能力，CCK-8法检查其对舌癌SCC-15细胞活力的影响。结果 靶向多功能纳米粒形态规则，呈球状。平均粒径（502.88±17.92） nm，平均电位（-11.5±3.15） mV。平均ELC为54.12%±1.74%，平均DLC为1.08 mg·mL-1。体外药物释放规律呈初期爆发性释放，接着是持续缓慢释放。其光热特性呈浓度相关，浓度越高温度越高。纳米粒可检测到明显光声信号和超声信号。体外靶向连接率89.99%。细胞活性实验结果表明，SINPs组在各个浓度下细胞活性无明显差异（P>0.05）。在浓度为150、200 μg·mL-1时，DOC-SINPs无论有无激光辐照均较SINPs有明显的细胞活性抑制（P<0.05）。结论 成功制备了集诊断与治疗一体的多功能纳米粒，具有超声/光声双模成像，同时具备化疗和光热治疗的抗肿瘤作用。.