{Reference Type}: Journal Article
{Title}: Targeting human urinary metabolome by LC-MS/MS: a review.
{Author}: Rodríguez-Morató J;Pozo ÓJ;Marcos J;
{Journal}: Bioanalysis
{Volume}: 10
{Issue}: 7
{Year}: Apr 2018 1
{Factor}: 2.695
{DOI}: 10.4155/bio-2017-0285
{Abstract}: Urine is a biological matrix that contains hundreds of metabolic end products which constitute the urinary metabolome. The development and advances on LC-MS/MS have revolutionized the analytical study of biomolecules by enabling their accurate identification and quantification in an unprecedented manner. Nowadays, LC-MS/MS is helping to unveil the complexity of urine metabolome, and the results obtained have multiple biomedical applications. This review focuses on the targeted LC-MS/MS analysis of the urine metabolome. In the first part, we describe general considerations (from sample collection to quantitation) required for a proper targeted metabolic analysis. In the second part, we address the urinary analysis and recent applications of four relevant families: amino acids, catecholamines, lipids and steroids.