{Reference Type}: Journal Article
{Title}: Identification of a novel human estrogen receptor-α splice variant able to enhance malignant biological behaviors of breast cancer cells.
{Author}: Zhu H;Huang Y;Su H;Ma Y;Tao Y;Liao DJ;Liu Y;Feng Z;
{Journal}: Oncol Lett
{Volume}: 15
{Issue}: 4
{Year}: Apr 2018
{Factor}: 3.111
{DOI}: 10.3892/ol.2018.7970
{Abstract}: Since the early 1990s, multiple human estrogen receptor-α (hER-α) splice variants have been identified, of which the majority contain ≥1 deleted exon, and some are expressed as proteins with modified functions from the wild-type 66 kDa hER-α (ER-α66). In the present study, a novel hER-α splice variant, ER-α30, was identified and cloned from clinical breast cancer tissue. The ER-α30 sequence lacked a ligand-binding domain and a ligand-dependent transcriptional activation domain but retained the N-terminal transcriptional activation domain, the DNA-binding domain and a partial hinge domain, and possesses a unique 10-amino-acid domain. The expression of ER-α30 was associated with ER-α66-negative and progesterone receptor-negative breast cancer. The 30 kDa protein was expressed in stably transfected MDA-MB-231 cells, and the overexpression of ER-α30 in MDA-MB-231 cells enhanced malignant biological behaviors, including cellular proliferation, migration and invasion in vitro. The results of the present study indicated that ER-α30 might represent a potential biomarker for breast cancer.